Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse 

Heat Shock Protein 25 (HSP 25) is constitutively expressed in smooth muscle. It is also expressed in fibroblasts following heat shock.

partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

The antibody also reacts with a dimer of the HSP 25 (approx. 58 kDa) appearing in preparations stored for prolonged periods prior to electrophoretic analysis. A major proteolytic fragment of 22 kDa may also be detected. The antibody suppresses the inhibition of actin elongation induced by the 25 kDa IAP. The antibody displays a punctate or fine fibrillary pattern of labeling throughout the cytoplasm in heat-shocked cells and co-localization with mitochondria. Reactivity has been observed with HSP 25 of various organs.

ascites fluid

IAP-28

antigen mol wt 25 kDa

15 mM sodium azide

human, bovine, rabbit, guinea pig, chicken, turkey, rat, mouse, frog

immunoblotting: 1:500 using turkey gizzard extract

immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle

indirect ELISA: suitable

indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts

radioimmunoassay: suitable

IgG1

mouse ... Hspb1(15507)

rat ... Hspb1(24471)

human ... HSPB1(3315)

dry ice

−20°C

Miron, T., et al. Eur. J. Biochem. 178, 543, (1988) abstract

Miron, T. J. Cell Biol. 114, 255, (1991) abstract

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