H1404
Sigma

Hepatocyte Growth Factor human

recombinant, expressed in Baculovirus infected High-5 cells, powder, cell culture tested

Synonym

Hepatopoietin A

HGF

Scatter Factor

MDL number

MFCD00217422

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Descriptions

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in the HGF responsive monkey epithelial cell line, 4MBr-5.

Biochem/physiol Actions

Hepatocyte growth factor is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Properties

recombinant

expressed in Baculovirus infected High-5 cells

assay

≥98% (SDS-PAGE)

form

powder

potency

0.01-0.1 ng/mL ED₅₀/EC₅₀

mol wt

mol wt ~80 kDa

total impurities

Endotoxin, tested

suitability

cell culture tested

Gene Information

human ... HGF(3082)

storage temp.

−20°C

References

Reference

Furlong, R.A., The biology of hepatocyte growth factor/scatter factor, et al., The biology of hepatocyte growth factor/scatter factor. Bioessays 14, 613-617, (1992) abstract

Nakamura, T., Structure and function of hepatocyte growth factor. Prog. Growth Factor Res. 3, 67-85, (1991) abstract

Safety

Safety Statements

22-24/25

WGK Germany