H2289
Sigma

Anti-Heat Shock Protein 27/25 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym

Anti-HSP27/25

Descriptions

Immunogen

synthetic peptide corresponding to amino acids 190-209 located at the C-terminus of mouse HSP27/25, conjugated to KLH. The sequence is identical in dog and Chinese hamster, highly conserved in rat HSP27 (85% identity), and to a lesser extent in human HSP27 (65%).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

mol wt

antigen mol wt 25 kDa (mouse)

antigen mol wt 27 kDa (human)

species reactivity

human, mouse, rat, canine

application(s)

immunoblotting: 0.5-1 μg/mL using whole extract of rat fibroblast Rat-1 cell line or Madin-Darby canine kidney (MDCK) cell line.

immunoblotting: 1-2 μg/mL using mouse brain extract (S1 fraction) or human HeLa cell line

indirect immunofluorescence: 4-8 μg/mL using MDCK cell line.

microarray: suitable

Gene Information

mouse ... Hspb1(15507)

human ... HSPB1(3315)

rat ... Hspb1(24471)

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany