H6409
Sigma

Monoclonal Anti-phospho-Histone H3 (pSer¹⁰) antibody produced in mouse

~2 mg/mL, clone H3-P, purified immunoglobulin, buffered aqueous solution

Synonym

Anti-Histone H3, phosphorylated (phosphoserine 10)

MDL number

MFCD01633984

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Descriptions

Immunogen

synthetic peptide corresponding to amino acids 1-18 (pSer¹⁰) of human histone H3.

Specificity

The antibody reacts with human histone H3, phosphorylated near its N-terminus (at Ser10), and does not detect the non-phosphorylated epitope.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

H3-P

form

buffered aqueous solution

mol wt

antigen mol wt 17 kDa

species reactivity

human

concentration

~2 mg/mL

application(s)

flow cytometry: 100-300 μg/mL using cultured human acute T cell leukemia Jurkat cells

immunoblotting: suitable

immunocytochemistry: suitable

indirect ELISA: suitable

microarray: suitable

isotype

IgG2a

Gene Information

human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Juan, G., et al., Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry 32, 71-77, (1998) abstract

Juan, G., et al., Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation. Exp. Cell Res. 246, 212-220, (1999) abstract

Safety

WGK Germany

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Related Categories

... Functional Profiling of Stem Cells > Antibodies for Epigenetics

... Antibodies in Chromatin Remodeling > Antibodies to Histone Modifications

... Antibodies for Cell Cycle > Antibodies to Nuclear Proteins

... Antibodies for Gene Regulation and Expression > Antibodies to Transcription Regulators