HIS-Select^® Cartridge 

The HIS-Select Cartridge is a prepacked ready-to-use cartridge (column) that contains 1.25 mL of HIS-Select Nickel Affinity Gel. This cartridge's Luer lock fittings make it suitable for Low Pressure Liquid Chromatography Systems (LPLC), Medium Pressure Liquid Chromatography Systems (MPLC), and for use with syringes.

The HIS-Select Cartridge has a storage buffer that contains 30% ethanol.

HIS-Select Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The HIS-Select Nickel Affinity gel is a proprietary, patent pending, quadridentate chelate on beaded agarose charged with nickel that is designed to specifically bind histidine containing proteins. The matrix for this affinity gel is 6% beaded agarose. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.

Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.

HIS-Select is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

≥15 mg/mL, gel binding capacity(as determined with an approx. 30 kDa histidine-tagged protein)

wet ice

2-8°C

UN 1170 3/PG 3

1

89 °F

32 °C