H8891
Sigma

Heparinase III from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% bovine serum albumin, 200-600 unit/mg solid

Synonym

Heparin Lyase III

Heparitinase from Flavobacterium heparinum

Heparitinase I

CAS Number

37290-86-1

Enzyme Commission (EC) Number

4.2.2.8 ( BRENDA | IUBMB )

MDL number

MFCD00131319

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Descriptions

Biochem/physiol Actions

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

Properties

mol wt

mol wt 70.8 kDa

storage temp.

−20°C

References

Reference

McLean, M.W. Proc. 9th Int. Symp. Glycoconjugates, B-41, (1987)

Lohse, D.L., and Linhardt, R.J. J. Biol. Chem. 267, 24347, (1992) abstract

Pojasek, K., et al., Histidine 295 and histidine 510 are crucial for the enzymatic degradation of heparan sulfate by heparinase III. Biochemistry 39, 4012-4019, (2000)

Safety

Safety Statements

22-24/25

WGK Germany

10-21