H9286
Sigma

Anti-acetyl-Histone H3 (Ac-Lys⁹) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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MDL number

MFCD02685921

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Descriptions

Immunogen

synthetic acetylated [Ac-Lys⁹] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

mol wt

antigen mol wt ~17 kDa

species reactivity

human, mouse, rat, bovine, chicken, frog, Drosophila, Caenorhabditis elegans

application(s)

immunoblotting: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate

microarray: suitable

Gene Information

human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)

rat ... H3f3b(117056)

mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)

shipped in

dry ice

storage temp.

−20°C