Monoclonal Anti-Integrin β1 antibody produced in mouse 

chicken integrin.¹

The antibody recognizes an epitope on the extracellular side of the avian integrin complex, and inhibits adhesion of embryo and tendon fibroblasts as well as skeletal myoblasts to both laminin and fibronectin. Under reducing and denaturing conditions using extracts of chicken embryo muscle cells, the antibody precipitates the β₁ class integrin heterodimer as a broad band of ~140 kDa. In the absence of reducing agents, the integrin β₁ subunit appears as bands at 100 kDa (immature form), 100 kDa (mature form), and 140 kDa (noncovalently associated α-subunits). The antibody may also be used for bioactivity studies in adhesion assays.

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.

purified immunoglobulin

W1B10

buffered aqueous solution

antigen mol wt 100-140 kDa

15 mM sodium azide

chicken

immunoblotting: 1:200 using denatured, non-reduced extract of cultured fibroblasts from chicken embryo lower leg

immunoprecipitation: suitable

indirect immunofluorescence: suitable

IgG1

chicken ... ITGB1(374058)

dry ice

−20°C

Hayahi, Y., et al. J. Cell Biol. 110, 175, (1990) abstract

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