Interferon α from rat 

The biological activity is determined in the cytopathic effect inhibition assay using rat epithelial-like (RATEC) cells with vesicular stomatitis virus (VSV) in the presence of 2 mM glutathione. The cytopathic effect of 50% is produced with ~1 unit/mL interferon. Since there is no international rat interferon α standard, units are determined with respect to the NIH international standard reference for mouse interferon α/β.

Lyophilized from a solution of 50 mM Tris-HCl, 20 mM citric acid, and 150 mM sodium chloride, pH 7, containing 200 μg/ml rat serum albumin and 125 mM trehalose.

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities. Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes) and ruminant embryos (τ subtypes). Interferon induces a variety of effects on target cells including antiviral, antiproliferative and immunomodulatory activities.

expressed in bacteria

≥95%

buffered aqueous solution

cell culture tested

rat ... Ifna_mapped(24480)

dry ice

−70°C

Rubinstein, S., et al., Convenient assay for interferons. J. Virol. 37, 755-758, (1981) abstract

Famillitti, P., et al., A convenient and rapid cytopathic effect inhibition assay for interferon. Meth. Enzymol. 78, 387-394, (1981) abstract

Pestka, S., Interferon standards and general abbreviations. Meth. Enzymol. 119, 14-23, (1986) abstract

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