JM109 Competent Cells, Uni-pack 

JM109 Competent Cells are packaged with sufficient reagents for 10 transformation reactions.

JM109 competent Escherichia coli is an all purpose cloning strain that is ideal for the generation of high quality plasmid DNA. JM109 is a K strain bacterium that carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation. The strain carries the hsdR17 genotype, which prevents cleavage of heter­ologous DNA by an endogenous endonuclease. JM109 cells also contain an F' episome carrying Δ(lacZ)M15 for blue/white screening via α-complementation with the amino terminus of β-galactosidase. The presence of the F' factor also allows growth of bacterio­phage M13 vectors for rescue of single stranded DNA.

Sigma's JM109 competent E. coli cells are grown and made chemically competent using an optimized procedure specific to the strain, followed by strain verification and efficiency testing. The cells are provided in frozen 50 μL aliquots for convenience. Each aliquot may be used for a single transformation. The cells are guaranteed to have a transformation efficiency of >1x10⁸ cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA.

dry ice

−70°C

S1797, SOC Medium

L7533, LB Agar EZMix^™ Powder

JM109 Competent Cells

pUC19 Control DNA