J4127
Sigma

Anti-Jagged 1 antibody produced in goat

affinity isolated antibody, lyophilized powder

MDL number

MFCD02096779

Descriptions

Antigen Background

Jagged-1 is a Notch ligand. The Notch signaling system regulates proliferation, differentiation, and apoptosis in many tissues, including hematopoietic tissue. Notch is a family of transmembrane receptors activated by ligands (Jagged-1, Jagged-2, and Delta-1) that are expressed on adjacent cells. Hematopoietic stem cells and early progenitors express Notch-1 and Notch-2, whereas primary stromal cultures have been shown to express Jagged-1, indicating that Jagged-1 may play a role in nurturing progenitor cells within the hematopoietic microenvironment. Cells engineered to express high levels of Jagged-1 caused a four-fold increase in formation of hematopoietic progenitor cell colonies. However, without the presence of other cytokines that stimulate proliferation and maturation, such as IL-3, IL-6, SCF and G-CSF, Jagged-1 actually decreased myeloid colony formation of CD34⁺ cells, suggesting that Jagged-1 modulates cytokine signals and helps preserve CD34⁺ cells in an immature state.

Immunogen

recombinant rat Jagged 1 extracellular domain, expressed in NSO cells.

Physical form

Lyophilized from 0.2 μm filtered solution of phosphate buffered saline.

Properties

antibody form

affinity isolated antibody

form

lyophilized powder

species reactivity

rat, human

application(s)

flow cytometry: 20 μg/mL using human Jagged 1 transfected FBM stromal cells

immunoblotting: 0.1-0.2 μg/mL

indirect ELISA: 0.5-1.0 μg/mL

neutralization: suitable

Gene Information

rat ... Jag1(29146)

human ... JAG1(182)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Miele, L., Osborne, B., Arbiter of differentiation and death: Notch signaling meets apoptosis. J. Cell Physiol. 181, 393, (1999) abstract

Walker, L., et al., The Notch Jagged pathway inhibits proliferation of human hematopoietic progenitors in vitro. Stem Cells 17, 162, (1999) abstract

Jones, P., et al., Stromal expression of Jagged-1 promotes colony formation by fetal hematopoietic progenitor cells. Blood 92, 1505, (1998) abstract

Varnum-Finney, B., The notch ligand, jagged-1 influences the development of primitive hematopoietic precursor cells. Blood 91, 4084, (1998) abstract