Lectin from Lycopersicon esculentum (tomato) 

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

LEA is not blood group specific, but has an affinity for N-acetyl-β-D-glucosamine oligomers. The lectin is a glycoprotein containing approx. equal amounts of protein and carbohydrate and is reported to inhibit the mitogenic activity of phytohemagglutinin from Phaseolus vulgaris.¹ This lectin binds endothelial cells and is used to visualize the microvasculature.

Sold on the basis of mg active conjugate.

Solution in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca²⁺, 0.08% sodium azide and 5 mg/mL β-clyclodextrin.

buffered aqueous solution

2-6 mol FITC per mol lectin

FITC conjugate

2-8°C

1. Nacbar, M.A., et al. J. Biol. Chem. 255, 2056, (1980)

Ezaki, T., et al., Time course of endothelial cell proliferation and microvascular remodeling in chronic inflammation. Am. J. Pathol. 158, 2043-2055, (2001)

Thurston, G., et al., Angiogenesis in mice with chronic airway inflammation: strain-dependent differences. Am. J. Pathol. 153, 1099-1112, (1998)

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