Lectin from Limulus polyphemus (horseshoe crab) 

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 7.4, containing 0.1 mM CaCl₂, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group B erythrocytes. Bacterial agglutination is determined from serial dilutions of a 1 mg/ml solution in 0.01 M Tris, 0.15 M NaCl, pH 7.4, 0.01M CaCl₂ with 1 mg/ml albumin and 0.92% TWEEN^®.

Agglutinates Staphylococcus aureus cells, as well as human and horse erythrocytes.
Major affinity is for N-acetylated D-hexosamines, but is reported to have anti-galactan specificity.¹

Package size based on protein content

Purified by affinity chromatography.

Lyophilized powder containing Tris succinate, NaCl and calcium acetate

lyophilized powder

<16 μg per mL agglutination activity (with blood group B erythrocytes)

−20°C

Gilbride, K.J. and Pistole, T.G. Prog. Clin. Biol. Res. 29, 525, (1979)

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