Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.
Biochem/physiol Actions
HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues.1 Conjugates are prepared from affinity purified lectin.
Reconstitution
Reconstitute with 5.0 mL of 0.9% saline solution or 0.01 M phosphate buffered saline for a working strength reagent for determining human Type A1 blood in tube or tile tests.