Escort^™ III Transfection Reagent 

ESCORT III is a liposomal formulation of a polycationic lipid and a neutral, non-transfecting lipid compound recommended for primary cells transfections. ESCORT III has been used to successfully transfect DNA into primary cells such as: primary human keratinocytes and tracheobronchial cells, primary quail myoblasts, primary sheep tracheobronchial cells, and primary hamster and rat hepatocytes. ESCORT III has also been shown to be effective for the transfection of HepG2, COS, NIH3T3, PC12 and Jurkat cells. No significant toxicity was observed at the concentrations used for optimal activity on the cell lines tested.
Optimal efficiencies are typically achieved using 1-4 μg ESCORT III per μg DNA on various cell lines tested in 35 mm dishes.

Do not freeze.

• Recommended for primary cell lines
• Low toxicity

Supplied as a sterile suspension in water at a concentration of 1 mg/ml.

Escort is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

1 mL sufficient for 250-1000 transfections

2-8°C

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