Lectin from Artocarpus integrifolia 

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Jacalin is extracted from Jack fruit seeds; a glycoprotein reported to bind human serum and secretory IgA.¹ Jacalin is not blood group specific.² The most effective inhibitor of hemagglutination is methyl α-D-galactopyranoside. The lectin is specific toward the Thomsen-Friedenreich (T) antigen, but has subtle differences in its combining site when compared to the site of Arachis hypogaea (peanut) agglutinin.³ Jacalin was also demonstrated to be a mitogen of T-lymphocytes.¹

Purified by affinity chromatography.

Contains phosphate buffer salts and NaCl

lyophilized powder

<10 μg per mL agglutination activity

Protein, ~70% biuret

2-8°C

1. Sastry, M. V., Analysis of saccharide binding to Artocarpus integrifolia lectin reveals specific recognition of T-antigen (β-D-Gal-(1→3)-D-GalNAc). J. Biol. Chem. 261, 11726, (1986)

2. Roque-Barreira, M.C., Campos-Neto, A., Jacalin: An IgA-binding Lectin. J. Immunol. 134, 1740, (1985)

3. Ahmed, H., Chatterjee, B.P. J. Immunol. Berlin , (1986) 5, 125

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