Application
Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Packaging
Package size based on protein content.
Preparation Note
Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O'Sullivan,1 which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Unit Definition
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Physical form
Contains citrate buffer
Related Information
Links
Page Options
