Lymphotoxin-α1/β2 human 

The biological activity is measured in a cytotoxicity assay using the TNF-susceptible mouse L929 cell line in the presence of actinomycin D.

Lymphotoxin-α (LT-α), also known as tumor necrosis factor-β (TNF-β), and lymphotoxin-β (LT-β) are members of the TNF family. Secreted LT-α complexes with the membrane-associated LT-β to generate two types of heterotrimers, LTα1/β2 and LTα2/β1. The predominant membrane-bound heterotrimer LTα1/β2 binds and activates only to the lymphotoxin β receptor (LT-βR). The other heterotrimer LTα2/β1 binds and activates TNF RI, TNF RII, and LT-βR. LT is critical for normal lymphoid organ development. LT is expressed by activated naive CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effector cells.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

expressed in Sf21 cells

≥95% (SDS-PAGE)

lyophilized powder

Endotoxin, tested

cell culture tested

human ... LTA(4049), LTB(4050)

−20°C

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Ware, C., et al., Thomson, A.W., ed., Tumor necrosis factor-related ligands and receptors. The Cytokine Handbook 3rd ed., San Diego, CA , (1998), 549

Rennert, P.D., Lymph node genesis is induced by signaling through the lymphotoxin-β receptor. Immunity 9, 71, (1998)

Browning, J.L., Characterization of lymphotoxin-α-β complexes on the surface of mouse lymphocytes. J. Immunol. 159, 3288, (1997)

Zhai, Y.F., et al., Light, a novel ligand for lymphotoxin-β receptor and tr2/hvem induces apoptosis and suppresses in vivo tumor formation via gene transfer. J. Clin. Invest. 102, 1142, (1998)

NWG