L5390
Sigma

Lectin from Erythrina cristagalli (coral tree)

lyophilized powder

Synonym

Erythrina cristagalli agglutinin

ECA

MDL number

MFCD00163567

Expand/Collapse All

Descriptions

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of trypsinized human blood group O erythrocytes after 1 hr at 25 °C.

Biochem/physiol Actions

ECA is not blood group specific, but has an affinity for D-galactose and D-galactosides. It is reported to be mitogenic for human peripheral blood T lymphocytes.¹

Preparation Note

Purified by affinity chromatography.

Properties

form

lyophilized powder

potency

<0.5 μg per mL agglutination activity

composition

Protein, ≥85% biuret

total impurities

salt, free

storage temp.