Lectin from Helix pomatia 

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues.¹ Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase Type VI (P8375) using a modification of the method of O'Sullivan,² which favors low molecular weight conjugates. Repurified by affinity chromatography after conjugation.

One unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Contains sodium chloride

lyophilized powder

50-120 units/mg protein

Protein, ≥90% Lowry

peroxidase conjugate

−20°C

1. Hammarstrom, S., Kabat, E. A. Biochemistry 10, 1684, (1971)

2. O'Sullivan, M.J., et al. FEBS Lett. 95, 311, (1978) abstract

Hammarstrom, S. Scand. J. Immunol. 1, 295, (1972)

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