L6632
Sigma

Lipoxidase from Glycine max (soybean)

Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein

Synonym

Linoleate:oxygen oxidoreductase

Lipoxygenase

CAS Number

9029-60-1

Enzyme Commission (EC) Number

1.13.11.12 ( BRENDA | IUBMB )

MDL number

MFCD00131527

EG/EC Number

232-853-1

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Descriptions

Analysis Note

Protein determined by biuret.

Application

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Preparation Note

Prepared by affinity chromatography.

Unit Definition

One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Suspension in 2.3 M (NH₄)₂SO₄ solution, pH approx. 6.0

Properties

type

Type V

form

ammonium sulfate suspension

mol wt

mol wt 108 kDa (two 54 kDa subunits)

storage temp.

2-8°C

References

Reference

Doderer, A., et al. Biochim. Biophys. Acta 1120, 97, (1992)

Rashbrook, L.C., et al. Biochem. Soc. Trans. 13, 233, (1985)

van Os, C.P.A. Biochim. Biophys. Acta 663, 177, (1981)

Safety

WGK Germany

Related Categories

... Arachidonic Acid Cascade > Enzymes

... General Metabolic Enzymes > Lipid Metabolism

... 1.x.x.x Oxidoreductases > 1.13.x.x Acting on single donors with incorporation of molecular oxygen

MSDS

Specification Sheet

Certificate of Analysis

More Information