Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg/ml solution. The activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.
Biochem/physiol Actions
SNA is isolated from the inner bark (bast tissue) of elder stems and branches.1 The lectin is not blood group specific but has an affinity for α-NeuNAc-[2→6]-Gal, α-NeuNAc-[2→6]-GalNAc, and, to a lesser extent, α-NeuNAc-[2→3]-Gal residues.2
Packaging
Package size based on protein content
Preparation Note
Affinity purified by a modification of Broekaert.3
Physical form
Lyophilized powder containing NaCl
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