Lectin from Maackia amurensis 

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2 of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hr incubation at 25 °C.

MAA consists of two molecular species, a strongly hemagglutinating hemagglutinin (MAH) and a strongly mitogenic hemagglutinin (MAL). The mitogenic activity of the latter is inhibited by α-sialyl-[2→3]-lactose. MAA interacts with sialic acid-containing glycoconjugate and may be useful for fractionation of sialylated oligosaccharides.

Purified by affinity chromatography using a modification of the method of Wang and Cummings.¹

lyophilized powder

<10 μg per mL agglutination activity

Protein, ≥85% Lowry

salt, essentially free

2-8°C

1. Wang, W.-C. and Cummings, R.D. J. Biol. Chem. 263, 4576, (1988) abstract

Kawaguchi, T., et al. J. Biol. Chem. 249, 2786, (1974)

Knibbs, R.N., et al. J. Biol. Chem. 266, 83, (1991)

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