Lectin from Ulex europaeus (gorse, furze) 

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Conjugates are prepared from affinity purified lectin.

Package size based on protein content.

Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.¹
Repurified by affinity chromatography after conjugation.

One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Contains sodium citrate buffer salts

lyophilized powder

<40 μg/mL agglutination activity

≥15 units/mg protein

Protein, ~80% Lowry

peroxidase conjugate

2-8°C

1. Avrameas, S. and Ternyck, T. Immunochemistry 8, 1175, (1971) abstract

Allen, H.J., Johnson, E.A.Z. Carbohydr. Res. 58, 253, (1977)

Horejsi, V., Kocourek, J. Biochim. Biophys. Acta 336, 329, (1974)

Matsumoto, I., Osawa, T. Biochim. Biophys. Acta 194, 180, (1969)

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