L8257
Sigma

Lactoperoxidase from bovine milk

lyophilized powder (essentially salt-free), ≥80 units/mg protein

Synonym

Donor:hydrogen peroxide oxidoreductase

Peroxidase

CAS Number

9003-99-0

Enzyme Commission (EC) Number

1.11.1.7 ( BRENDA | IUBMB )

MDL number

MFCD00131484

EG/EC Number

232-668-6

Expand/Collapse All

Descriptions

Analysis Note

Protein determined by Lowry method.

Biochem/physiol Actions

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Other Notes

Note: Using 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt as substrate, two or three times the activity is observed.

Unit Definition

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Properties

form

lyophilized powder (essentially salt-free)

absorbance ratio

A_412/280 nm 0.88-0.95

Gene Information

cow ... LPO(280844)

storage temp.

−20°C

References

Reference

Gladek, A. and Modarski, M., Enzymatic method of incorporation of ¹²⁵I into DNA. Arch. Immunol. Ther. Exp. 31, 541, (1983)

Morrison, M., Lactoperoxidase catalyzed iododination as a tool for investigation of proteins. Meth. Enzymol. 70, 214-220, (1980)

David, G.S. and Reisfeld, R.A., Protein iodination with solid state lactoperoxidase. Biochemistry 13, 1014, (1974)

Safety

WGK Germany

10-21