Lectin from Helix pomatia 

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues.¹ Conjugates are prepared from affinity purified lectin.

Suspension in 0.5 M NaCl, 10 mM phosphate buffer, pH 7.3, and 0.02% sodium azide

saline suspension

1-3 mg lectin per mL

cross-linked 4% beaded agarose

agarose conjugate

2-8°C

1. Hammarstrom, S., Kabat, E. A. Biochemistry 10, 1684, (1971)

Hammarstrom, S. Scand. J. Immunol. 1, 295, (1972)

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