Lectin from Phaseolus vulgaris (red kidney bean) 

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.^1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.³ PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.⁴ PHA-M is the mucoprotein form.⁴ Conjugates are prepared from the corresponding purified lectins.

lyophilized powder

<62.5 μg per mL agglutination activity

<10 μg/mL mitogenic activity

salt, free

2-8°C

1. Yachnin, A. and Svenson, R.H. Immunology 22, 871, (1972)

2. Felsted, et al. J. Biol. Chem. 252, 2967, (1977)

3. Kornfeld, R. and Kornfeld, S. J. Biol. Chem. 245, 2536, (1970)

4. Rigas, D.A. and Osgood, E.E. J. Biol. Chem. 212, 607, (1955)

TJ3470000