Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Biochem/physiol Actions
Tetragonolobus purpureas agglutinin has anti-H blood group specificity. The lectin has an affinity for α-L-fucosyl residues and an unusually high affinity for α-L-fucose residues on type II chain blood group oligosaccharides.
Preparation Note
Conjugates are prepared from affinity purified lectin.
Purified by affinity chromatography.
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