M-MLV Reverse Transcriptase 

M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. M-MLV is used for the preparation of cDNA libraries or for first strand cDNA synthesis for use in RT-PCR reactions.

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

expressed in Escherichia coli

200 units/μL

wet ice

−20°C

Howland, P., et al., Positive- and negative-acting promoter sequences regulate cell type-specific expression of the rat synapsin I gene. Brain Res. Mol. Brain Res. 11, 345-353, (1990)

Gerard, G.F. and D'Alessio, J.M., Buwell, M., Enzymes of Molecular Biology. Methods Mol. Biol. Totowa, N.J. 16, 73, (1993)

Gerard, G.F., et al., Influence on stability in Escherichia coli of the carboxy-terminal structure of cloned Moloney murine leukemia virus reverse transcriptase. DNA 5, 271-279, (1986) abstract

3

N8878, Nalidixic acid

N4382, Nalidixic acid sodium salt

R5277, RT-PCR Protocols