Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600) 

The ultralow range marker is designed for use in the Tris-Tricine SDS-PAGE method when stained with Brilliant blue G (non-methanol based). Glutaraldehyde fixing is recommended with the ultralow marker in order to keep the two smallest proteins fixed in the gel. The ultralow range marker is supplied as a liquid, which must be diluted (1:20) with the supplied sample buffer prior to loading.

Solution in 0.1 M Tris-HCl, pH approx. 8.5, with 4 mM EDTA, 3 mM sodium azide and 40% glycerol

1 vial sufficient for 400 applications on large gels (16 × 18 cm)

1 vial sufficient for 800 mini-gels (10 × 10 cm)

vial of 200 μL

−20°C

Schagger, H. and von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379, (1987) abstract

1

Triose Phosphate Isomerase, Rabbit Muscle 26,600 Da MW

Myoglobin, Horse Heart, 17,000 Da MW

α-Lactalbumin, Bovine Milk, 14,200 Da MW

Aprotinin, Bovine Lung, 6,500 Da MW

Insulin Chain B, Oxidized, Bovine, 3,496 Da MW

Bradykinin, 1,060 Da MW

Tris-Tricine sample buffer 10 mL