M3557
Sigma

Monoclonal Anti-MAP Kinase, Monophosphorylated Threonine antibody produced in mouse

~2 mg/mL, clone ERK-YNP, purified immunoglobulin, buffered aqueous solution

Synonym

Anti-pT-ERK

MDL number

MFCD01633612

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Descriptions

Immunogen

synthetic peptide HTGFLTEYVAT, corresponding to the non-phosphorylated form of ERK-activation loop.

Preparation Note

Prepared from a culture supernatant of bioreactor grown hybridoma.

Specificity

The antibody reacts with the monophosphorylated threonine MAP kinase (ERK-1 and ERK-2) proteins and weaker with the non-phosphorylated forms of MAP kinases. It does not recognize the diphosphorylated, and the monophosphorylated tyrosine forms of the ERK/MAPK proteins, or the JNK and p38 MAPK. The epitope recognized by the antibody contains the threonine residue within the regulatory site of MAP kinase (e.g.,Thr¹⁸³ in ERK-2).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

ERK-YNP

form

buffered aqueous solution

mol wt

antigen, ERK-1 mol wt 44 kDa

antigen, ERK-2 mol wt 42 kDa

species reactivity

human, rat, mouse

concentration

~2 mg/mL

application(s)

capture ELISA: suitable

immunoblotting: 5-20 μg/mL using rat brain extract

immunocytochemistry: suitable

microarray: suitable

isotype

IgG1

Gene Information

mouse ... Mapk1(26413), Mapk3(26417)

rat ... Mapk1(116590), Mapk3(50689)

human ... MAPK1(5594), MAPK3(5595)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Yao, Z, et al., Detection of partially phosphorylated forms of ERK by monoclonal antibodies reveals spatial regulation of ERK activity by phosphatases. FEBS Lett. 468, 37-42, (2000) abstract

Safety

WGK Germany