Monoclonal Anti-MAP Kinase, Non-Phosphorylated ERK antibody produced in mouse 

synthetic peptide HTGFLTEYVAT, corresponding to the non-phosphorylated form of ERK-activation loop.

Prepared from a culture supernatant of bioreactor grown hybridoma

The antibody reacts specifically with the non-phosphorylated, non-activated form of MAP kinase (ERK-1 and ERK-2). Weak cross-reaction is observed with monophosphorylated (threonine or tyrosine), but not with diphosphorylated peptides of MAPK. The antibody does not recognize JNK or p38 MAPK. The epitope recognized by the antibody contains non-phosphorylated threonine 183 and tyrosine 185 which resides within the ERK-activation loop (e.g., amino acids 178-188 in ERK-2).

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

purified immunoglobulin

ERK-NP2

buffered aqueous solution

antigen, ERK-1 mol wt 44 kDa

antigen, ERK-2 mol wt 42 kDa

human, rat

~2 mg/mL

immunoblotting: 5-25 μg/mL using rat brain extract

immunocytochemistry: suitable

indirect ELISA: suitable

microarray: suitable

IgG1

rat ... Mapk1(116590), Mapk3(50689)

human ... MAPK1(5594), MAPK3(5595)

dry ice

−20°C

3

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