M4308
Sigma

Monoclonal Anti-MDM2 antibody produced in mouse

clone SMP14, ascites fluid

MDL number

MFCD03792424

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Descriptions

Immunogen

synthetic peptide corresponding to amino acids 154-167 of human MDM2 conjugated to KLH.

Specificity

Detects the MDM2 protein alone or in complex with E2F1, p53, and retinoblastoma proteins. A slight cross-reactivity is observed with cytokeratins 6, 14, and 16.

Properties

antibody form

ascites fluid

clone

SMP14

mol wt

antigen mol wt ~90 kDa (additional band of approx. 55 kDa)

contains

15 mM sodium azide

species reactivity

human, rat, mouse (weak)

application(s)

immunoblotting: 1:1,000 using a whole extract of transfected 293T (human embryonal kidney) cells expressing human MDM2

immunocytochemistry: suitable

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

immunohistochemistry (frozen sections): suitable

immunoprecipitation: suitable

indirect ELISA: suitable

microarray: suitable

isotype

IgG1

Gene Information

mouse ... Mdm2(17246)

rat ... Mdm2(246362)

human ... MDM2(4193)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Picksley, S.M., Immunochemical analysis of the interaction of p53 with MDM2; fine mapping of the MDM2 binding site on p53 using synthetic peptides. Oncogene 9, 2523, (1994) abstract

Hickman, E.S., et al., Cells expressing HPV16 E7 continue cell cycle progression following DNA damage induced p53 activation. Oncogene 9, 2177-2181, (1994) abstract

Martin, K., et al., Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein. Nature 375, 691-694, (1995) abstract

Safety

WGK Germany

Related Products

Array

XP725, Panorama^® Antibody Microarray - XPRESS Profiler725 Kit

Related Categories

... Antibodies to Protein Degradation > Antibodies to E1,E2,E3 Ligase

... Antibodies for Cell Cycle > Antibodies to Nuclear Proteins

... Antibodies for Cell Cycle > Antibodies to p53 and Related Proteins

... M > Other

... Tumor Growth Regulation > Protooncogene Products