M4819
Sigma

Anti-methyl-Histone H3 (Me-Lys⁴) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Descriptions

Immunogen

synthetic methylated peptide corresponding to amino acid 1-8 (Me-Lys⁴) at the N-terminus of human histone H3, conjugated to KLH. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, C. elegans, tetrahymena, and Arabidopsis thaliana histone H3.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

mol wt

antigen mol wt 17 kDa

species reactivity

human, mouse, rat, bovine, chicken, frog, Drosophila, Caenorhabditis elegans, Arabidopsis thaliana

application(s)

immunoblotting: 0.5-1 μg/mL using whole cell extract of human HeLa cells, human Jurkat cells, or mouse NIH3T3 cells

Gene Information

human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)

mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)

rat ... H3f3b(117056)

shipped in

dry ice

storage temp.

−20°C