Silver Stain Molecular Weight Marker (M.W. 6,500-180,000) 

Designed for molecular weight determinations on silver stained SDS-PAGE (Laemmli) gels. The proteins supplied in these markers cover the range of molecular weights common to most proteins or their subunits. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity with low background after electrophoresis and staining using either ProteoSilver™ (PROTSIL1) or ProteoSilver™ Plus (PROTSIL2).

Will yield 9 bands with silver staining:
• α₂-Macroglobulin, 180,000 Da
• β-Galactosidase, E. coli, 116,000 Da
• Phosphorylase b, Rabbit Muscle, 97,400 Da
• Serum Albumin, Bovine, 66,000 Da
• Fumarase, Porcine Heart, 48,500 Da
• Carbonic Anhydrase, Bovine Erythrocytes, 29,000 Da
• β-Lactoglobulin, Bovine Milk, 18,400 Da
• α-Lactalbumin, Bovine Milk, 14,200 Da
• Aprotinin, Bovine Lung, 6,500 Da

Vial contains 200 μl of protein markers. Supplied with one vial of 2× Laemmli sample buffer (S3401).

Dilute the concentrate between 1:15 and 1:30 depending on the amount of protein loaded in the other lanes. Recommended loading volumes are 10 μl per well for mini gels (10 × 10 cm) or 20 μl per well for standard format gels (16 × 18 cm).

Solution in 300 mM NaCl, 100 mM DTT, 3 mM NaN₃, and 50% glycerol

aqueous glycerol solution

sufficient for 300 standard gels (16 × 18 cm)

1 vial sufficient for 600 mini-gel applications

mol wt 6,500-180,000 Da

dry ice

−20°C

Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970) abstract

Heukeshoven, J. and Dernick, R., Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6, 103-112, (1985)

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