M6908
Sigma

Myeloperoxidase from human leukocytes

lyophilized powder, ≥50 units/mg protein

Synonym

MPO

Peroxidase, myelo

CAS Number

9003-99-0

Enzyme Commission (EC) Number

1.11.1.7 ( BRENDA | IUBMB )

MDL number

MFCD00131641

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Descriptions

Analysis Note

Source material tested and found negative for antibody to HIV and for HBSAG.

Biochem/physiol Actions

Catalyzes oxidations by hydrogen peroxide, including cytotoxic actions on bacteria, fungi, and tumor cells. Also catalyzes cross-linking of immune complexes and inactivates chemotactic factors.

Other Notes

Lysosomal heme-containing protein.

Unit Definition

One unit will produce an increase in absorbance (A₄₇₀) of 1.0 per min at pH 7.0 and 25 °C, calculated from the initial rate of reaction using guaiacol as substrate. Total reaction volume: 3.035 mL.

Physical form

Lyophilized from 0.02 M sodium acetate buffer, pH 6.0.

Properties

form

lyophilized powder

solubility

H₂O: soluble

aqueous buffers such as 0.02 M sodium acetate pH 6.0: Freezing of the enzyme in solution may result in a substantial loss of activity.soluble

Gene Information

human ... MPO(4353)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Clark, R.A., et al., Peroxidase-H₂O-halide system: Cytotoxic effect on mammalian tumor cells. Blood 45, 161, (1975) abstract

Clark, R.A., and Szot, S., Chemotactic factor inactivation by stimulated human neutrophils mediated by myeloperoxidase-catalyzed methionine oxidation. J. Immunol. 128, 1507-1513, (1982) abstract

Lehrer, R.I., The fungicidal mechanisms of human monocytes. I. Evidence for myeloperoxidase-linked and myeloperoxidase-independent candidacidal mechanisms. J. Clin. Invest. 55, 338-346, (1975) abstract

Uesugi, M., et al., Covalent cross-linking of immune complexes by oxygen radicals and nitrite. J. Immunol. 161, 1422-1427, (1998) abstract

Jasin, H.E., Oxidative cross-linking of immune complexes by human polymorphonuclear leukocytes. J. Clin. Invest. 81, 6-15, (1988) abstract

Odell, E.W., and Segal, A.W., The bactericidal effects of the respiratory burst and the myeloperoxidase system isolated in neutrophil cytoplasts. Biochim. Biophys. Acta 97, 266-274, (1988)

Safety

Hazard Codes

WGK Germany