1. Gene Identification Classical mutagenesis in higher plants,
2. Plant transformation,
3. T-DNA tagging,
4. Transposion tagging methods in heterologous plants,
5. Cloning plant genes by genomic subtraction,
6. Gene Organization Approaches to gene mapping,
7. Arabidopsis YAC, BAC and cosmid libraries,
8. In situ hybridization to plant chromosomes and DNA fibres,
9. Library Screening and cDNA Isolation PCR cloning approaches,
10. Western and southwestern library screens,
11. Complementation Cloning,