M7318
Sigma

Anti-MBD2a,b (RA-18) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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MDL number

MFCD06798469

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Descriptions

Immunogen

synthetic peptide corresponding to amino acids 294-411 of human MBD2a conjugated to KLH via an N-terminal added lysine residue. This sequence is conserved in MBD2a and MBD2b of human origin, and is not found in MBD3.

Specificity

The antibody recognizes MBD2a (~45 kDa) and MBD2b (~29 kDa). In immunoblotting, an unidentified band at ~43 kDa may also be detected.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

species reactivity

human, mouse

application(s)

immunoblotting: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells

indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells

microarray: suitable

Gene Information

mouse ... Mbd2(17191)

human ... MBD2(8932)

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany

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M7318 - Datasheet (33kb)

MSDS

Certificate of Analysis

More Information