M7802
Sigma

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

MDL number

MFCD01633612

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Descriptions

Immunogen

synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Specificity

The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr¹⁸³ in ERK-2).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

ERK-PT115

form

buffered aqueous solution

mol wt

antigen, ERK-1 mol wt 44 kDa

antigen, ERK-2 mol wt 42 kDa

species reactivity

human, rat

concentration

~2 mg/mL

application(s)

capture ELISA: suitable

immunoblotting: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol

immunocytochemistry: suitable

microarray: suitable

isotype

IgG1

Gene Information

rat ... Mapk1(116590), Mapk3(50689)

human ... MAPK1(5594), MAPK3(5595)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Yao, Z, et al., Detection of partially phosphorylated forms of ERK by monoclonal antibodies reveals spatial regulation of ERK activity by phosphatases. FEBS Lett. 468, 37-42, (2000) abstract

Safety

WGK Germany

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Related Categories

... Hematopoietic Stem Cell Characterization/Differentiation > Antibodies to Gene Expression/Regulation

... Clone Index > E

... Serine/Threonine Kinase Antibodies > Mitogen-Activated Protein Kinase (MAPK)

... M > Other

M7802 - Datasheet (32kb)

MSDS

Certificate of Analysis

More Information