M8159
Sigma

Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody produced in mouse

clone MAPK-YT, ascites fluid

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Synonym

Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)

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Descriptions

Immunogen

synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop.

Specificity

The antibody reacts specifically with the diphosphorylated form of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase.

Properties

antibody form

ascites fluid

clone

MAPK-YT

mol wt

antigen ERK-1 mol wt 44 kDa

antigen ERK-2 mol wt 42 kDa

contains

15 mM sodium azide

species reactivity

human, rat, mouse, Drosophila, yeast, bovine, hamster, Xenopus, Caenorhabditis elegans

application(s)

immunoblotting: 1:10,000 using rat brain extract

immunocytochemistry: suitable

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

immunoprecipitation: suitable

indirect ELISA: suitable

isotype

IgG1

Gene Information

mouse ... Mapk1(26413), Mapk3(26417)

rat ... Mapk1(116590), Mapk3(50689)

human ... MAPK1(5594), MAPK3(5595)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Yao, Z., et al., Detection of partially phosphorylated forms of ERK by monoclonal antibodies reveals spatial regulation of ERK activity by phosphatases. FEBS Lett. 468, 37-42, (2000)

Yung, Y., et al., Detection of ERK activation by a novel monoclonal antibody. FEBS Lett. 408, 292-296, (1997)

Safety

WGK Germany