Mannitol Dehydrogenase from Leuconostoc mesenteroides 

Assayed according to the method of Yamanaka, Meth. Enzymol., 41(B), 138 (1975).

This preparation is useful in the determination of mannitol in urine.

Partially purified

One unit will reduce 1.0 μmole of D-fructose per min in the presence of NADH at pH 5.3 at 30°C. One unit of enzyme, as defined above, is equivalent to approximately 1.8 units of enzyme, as described by Lunn et al., one unit will oxidize 1.0 μmole of D-mannitol per min in the presence of NAD at pH 8.6 at 40°C.

Lyophilized powder containing buffer salts, potassium phosphate, and dithiothreitol

lyophilized powder

Protein, ≥30% biuret

−20°C

Lunn, P.G., et al. Clin. Chim. Acta 183, 163, (1989) abstract

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