Mitochondria Isolation Kit 

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

• Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
• Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
• Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
• Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

sufficient for 10-20 g (animal tissue)

sufficient for 50 assays (2 mL)

2 yr

dry ice

−20°C

UN 3316 9/PG 2

CS0720, Citrate Synthase Assay Kit

CS0760, Isolated Mitochondria Staining Kit - Determine integrity of inner mitochondrial membrane.

CYTOCOX1, Cytochrome c Oxidase Assay Kit - For the determination of cytochrome c oxidase activity in mitochondrial soluble and membrane bound samples.

MITOISO2, Mitochondria Isolation Kit - For the isolation of enriched mitochondrial fraction from cells.

MITOISO3, Yeast Mitochondria Isolation Kit - For the isolation of enriched mitochondrial fraction from yeast.

Extraction buffer A 5X 50 mL

Albumin solution from bovine serum 10 mL

Extraction buffer B, 5× 50 mL

JC-1 Stain 25 μg

JC-1 Assay buffer 5X 25 mL

Storage buffer 5X 25 mL

Trypsin from bovine pancreas, lyophilized powder, ≥7,500 BAEE units/mg solid