Neurotrophin-4 human 

The biological activity is measured in a cell proliferation assay using a TrkB-transfected cell line BaF-TrkB-BD.

Neurotrophin-4 (also called NT-4/5 or NT-5) is a 14 kDa member of the neurotrophin family. NT-4 shares 95% sequence homology between human and rat. NT-4 from human, rat, and Xenopus are active on chick dorsal root ganglia. NT-4 is expressed in many tissues and major brain regions. NT-4 promotes survival and differentiation of various cells in culture including spinal neuons, basal forebrain cholinergic neurons, hippocampal neurons, cerebellar granule cells, embryonic dopaminergic neruons of mesencephalon, noradrenergic neurons of the locus coeruleus, dopaminergic, GABAergic, and serotoninergic neurons of the substantia nigra, and embryonic trigeminal and jugular neurons.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 50 μg bovine serum albumin per 1 μg of cytokine.

expressed in Sf21 cells

≥97% (SDS-PAGE)

lyophilized powder

mol wt 14 kDa

Endotoxin, tested

cell culture tested

human ... NTF5(4909)

−20°C

Timmusk, T., et al., Widespread and developmentally regulated expression of neurotrophin-4 mRNA in rat brain and peripheral tissues. Eur. J. Neurosci. 5, 605, (1993) abstract

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