N214
Sigma

Neutrophil Activating Peptide-2 human

>97% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested

Synonym

NAP-2

MDL number

MFCD00241917

Expand/Collapse All

Descriptions

Analysis Note

The biological activity is measured by the induction of myeloperoxidase release from cytochalasin B treated human neutrophils.¹

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Biochem/physiol Actions

Neutrophil Activating Peptide-2, a member of the CXC chemokine family, chemoattracts and activates neutrophils and also binds CXCR-2. NAP-2 is proteolytically processed C-terminal fragments of platelet basic protein (PBP), which is found in the α-granules of human platelets.

Properties

recombinant

expressed in Escherichia coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

total impurities

Endotoxin, tested

suitability

cell culture tested

Gene Information

human ... PPBP(5473)

storage temp.

−20°C

References

Cited Reference

1. Schröder, J.M., Purification and partial biochemical characterization of a human monocyte-derived neutrophil-activating peptide that lacks interleukin 1 activity. J. Immunol. 139, 3474, (1987) abstract

Reference

Lida, N., et al., Leukocyte-derived growth factor links the PDGF and CXC chemokine families of peptides. FASEB J. 10, 1336-1345, (1996) abstract

Proudfoot, A.E., Structure and bioactivity of recombinant human CTAP-III and NAP-2. J. Protein Chem. 16, 37, (1997) abstract

Safety

Hazard Codes

Risk Statements

23/24/25-36/37/38

Safety Statements

26-36/37/39-45