Monoclonal Anti-Nitric Oxide Synthase, Universal antibody produced in mouse 

bovine cerebellum NOS.

The antibody reacts with iNOS, eNOS and nNOS from rat and bovine, and with iNOS in induced RAW 264.7 mouse macrophages. In immunoblotting, the antibody detects a band at 130 kDa representing iNOS in samples first induced with IFN (interferon) and LPS (lipopolysaccharides). It also detects a band at 155 kDa in tissues expressing nNOS and a band at 140 kDa in tissues expressing eNOS. The antibody may be used to localize and detect the three isoforms of nitric oxide synthase (NOS): neuronal or brain (nNOS or bNOS), endothelial (eNOS) and inducible (iNOS).

Solution in phosphate buffered saline, containing 0.05% sodium azide.

ascites fluid

NOS-3F7-B11-B5

rat, mouse, bovine

immunoblotting: 1:250

immunohistochemistry (frozen sections): 1:100

IgM

mouse ... Nos1(18125)

rat ... Nos1(24598)

dry ice

−20°C

Rengasamy, A., et al., Immunohistochemical demonstration of a paracrine role of nitric oxide in bronchial function. Am. J. Physiol. 267, L704, (1994) abstract

Xue, C., et al., Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia. Am. J. Physiol. 267, L667, (1994) abstract

NWG