N2912
Sigma

Monoclonal Anti-Neurofilament 160/200 antibody produced in mouse

~2 mg/mL, clone RMdO20, purified immunoglobulin, buffered aqueous solution

MDL number

MFCD00164708

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Descriptions

Immunogen

purified mid-size rat neurofilament (NF-M) subunit.

Specificity

Recognizes mainly the non-phosphorylated form of NF-M and NF-H (NF-M/H, ~160 and 200 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

RMdO20

form

buffered aqueous solution

species reactivity

human, mouse, rat

concentration

~2 mg/mL

application(s)

immunoblotting: 1-2 μg/mL using extracts of rat brain S1 fraction.

immunocytochemistry: suitable

immunohistochemistry: suitable

microarray: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

References

Reference

Lee, V.M.Y., et al., Monoclonal antibodies distinguish several differentially phosphorylated states of the two largest rat neurofilament subunits (NF-H and NF-M) and demonstrate their existence in the normal nervous system of adult rats. J. Neurosci. 7, 3474-3488, (1987) abstract

Yu, W., et al., Depletion of a microtubule-associated motor protein induces the loss of dendritic identity. J. Neurosci. 20, 5782-5791, (2000) abstract

Galvin, J.E., et al., Neurodegeneration with brain iron accumulation, type 1 is characterized by α-, β-, and γ-synuclein neuropathology. Am. J. Pathol. 157, 361-368, (2000) abstract

Fung, K.M., et al., A novel modification of the avidin-biotin complex method for immunohistochemical studies of transgenic mice with murine monoclonal antibodies. J. Histochem. Cytochem. 40, 1319-1328 , (1992) abstract

Safety

WGK Germany