N3033
Sigma

Nitric Oxide Synthase, Neuronal from rat

≥95%, recombinant, expressed in Sf9 cells, buffered aqueous solution

Synonym

bNOS

ncNOS

Neuronal Nitric Oxide Synthase

NOS I

MDL number

MFCD00213013

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Descriptions

Biochem/physiol Actions

NOS is responsible for the biosynthesis of nitric oxide from L-arginine. nNOS is calcium/calmodulin dependent and has a K_m = 2.0 μM for L-arginine.

Unit Definition

One unit of enzyme produces 1 nmole of nitric oxide per minute at 37�°C in 50 mM HEPES, pH 7.4, containing 5 μM oxyhemoglobin, 1 mM CaCl₂, 20 μg/ml calmodulin, 0.1 mM NADPH, 50 μM arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.

Physical form

Solution in 50 mM HEPES, pH 7.4, with 20% glycerol, 100 mM NaCl and 10 μM tetrahydrobiopterin.

Properties

recombinant

expressed in Sf9 cells

assay

≥95%

form

buffered aqueous solution

activity

≥300 units/mg protein

mol wt

mol wt 150 (kDa/subunit, homodimer)

Gene Information

rat ... Nos1(24598)

shipped in

dry ice

storage temp.

−70°C

References

Reference

Hevel, J.M., and Marletta, M.A., Nitric-oxide synthase assays. Meth. Enzymol. 233, 250, (1994)

Richards, M.K., and Marletta, M.A., Characterization of neuronal nitric oxide synthase and a C415 mutant, purified from a baculovirus overexpression system. Biochemistry 33, 14723, (1994)

Knowles, R.G., and Moncada, S., Nitric oxide synthases in mammals. Biochem. J. 298, 249, (1994)

Safety

WGK Germany