N3393
Sigma

Naphthol Blue Black

for electrophoresis

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Synonym

Acid Black 1

Amido Black 10B

Buffalo Black NBR

Molecular Formula

C₂₂H₁₄N₆Na₂O₉S₂

Molecular Weight

616.49

CAS Number

1064-48-8

Colour Index Number

20470

Beilstein Registry Number

5374263

MDL number

MFCD00004017

PubChem Substance ID

24897642

EG/EC Number

213-903-1

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Descriptions

Application

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

Properties

grade

for electrophoresis

composition

Dye content, ~85%

total impurities

≤10% water

References

Reference

De Vito, E. and Santome, J., Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate. Experientia 22, 124-125, (1966) abstract

Towbin, H., et al., Electrophoretic transfer of proteins form polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354, (1979) abstract

Wilson, C.M., Staining of proteins on gels: comparisons of dyes and procedures. Meth. Enzymol. 91, 236-247, (1983) abstract

Beilstein

Beil. 16,IV,622

reference

Corp MSDS 1 (2), 2505:A / FT-IR 2 (3), 4108:A / FT-IR 1 (2), 990:C / IR-Spectra (3), 1464:B / RegBook 1 (2), 2759:O / Sigma FT-IR 1 (2), 181:C / Stains and Dyes Ref, 496 / Structure Index 1, 437:B:8

Safety

Hazard Codes

Risk Statements

36/37/38

Safety Statements

WGK Germany

RTECS

QJ6196000