Nuclear Transport Factor 2 human 

Measured in a binding assay of Ran to NTF2.

Lyophilized powder containing Hepes buffer salts, potassium acetate, magnesium acetate, EGTA, and sucrose.

Mediates the nuclear import of RanGDP, driving the nuclear accumulation of Ran. This process is essential for maintaining the cellular nuclear transport and for cell viability.

Mediates the nuclear import of RanGDP, driving the nuclear accumulation of Ran. This process is essential for maintaining the cellular nuclear transport and for cell viability. NTF2, which possesses non-overlapping binding sites for both RanGDP and nucleoporin FxFG repeats, binds RanGDP in the cytoplasm and carries it via the nuclear pore complex (NPC) by binding to the FxFG-repeats, which allows rapid attachment and detachment of NTF2 to the nucleoporins while it passes through the NPC. At the nuclear side of the NPC, the release of RanGDP from NTF2 may be promoted by dissociation of the NTF2 to its monomers.

expressed in Escherichia coli

>90% (SDS-PAGE)

lyophilized powder

human ... NUTF2(10204)

wet ice

−20°C

Paschal, B.M., and Gerace, L. J. Cell Biol. 129, 925-937, (1995)

Quimby, B.B., et al. J. Biol. Chem. 276, 38820-38829, (2001)

Stewart, M. Cell Struct. Funct. 25, 217-225, (2000)

Chaillan-Huntington, C., et al. J. Mol. Biol. 314, 465-477, (2002)

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