N5408
Sigma

Monoclonal Anti-Nerve Growth Factor Receptor antibody produced in mouse

clone ME20.4, ascites fluid

Synonym

Anti-NGFR p75

MDL number

MFCD00282384

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Descriptions

Immunogen

human melanoma cell line WM245.

Specificity

Recognizes the low affinity human NGFR p75 (also known as gp75^NGFR , p75^NGFR and p75^NTR).¹ The epitope recognized by the antibody exists within the third or fourth cysteine-rich repeats of the NGFR molecule. May be used in various immunoassays including inhibition of NGF binding to p75-NGFR and as a cholinergic immunotoxin.

Properties

antibody form

ascites fluid

clone

ME20.4

mol wt

antigen mol wt 75 kDa

contains

15 mM sodium azide

species reactivity

human, monkey, pig, feline, racoon

does not react with

rat, chicken, mouse

application(s)

flow cytometry: suitable

immunoblotting: suitable

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tongue

immunohistochemistry (frozen sections): suitable

immunoprecipitation: suitable

indirect ELISA: suitable

microarray: suitable

neutralization: suitable

radioimmunoassay: suitable

isotype

IgG1

Gene Information

human ... NGFR(4804)

shipped in

dry ice

storage temp.

−20°C

References

Cited Reference

1. Ross, A.H., et al., Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. Proc. Natl. Acad. Sci. USA 81, 6681-6685, (1984) abstract

Reference

Dominici, C., et al., Immunohistochemical detection of p140trkA and p75LNGFR neurotrophin receptors in neuroblastoma. J. Neurooncol. 31, 57-64, (1997) abstract

Fine, A., et al., Learning impairments following injection of a selective cholinergic immunotoxin ME20.4 IgG-saporin into the basal nucleus of Meynert in monkeys. Neuroscience 81, 331, (1997) abstract

Yan, H., and Chao, M.V., Disruption of cysteine-rich repeats of the p75 nerve growth factor receptor leads to loss of ligand binding. J. Biol. Chem. 266, 12099, (1991) abstract

Vissavajjhala, P., Structural domains of the extracellular domain of human nerve growth factor receptor detected by partial proteolysis. Arch. Biochem. Biophys. 294, 244, (1992) abstract

Safety

WGK Germany

Related product

N5788, Anti-Nerve Growth Factor Receptor/TNFRSF16 antibody produced in goat

N3908, Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in rabbit

N6163, Anti-Nerve Growth Factor Receptor/TNFRSF16 antibody produced in goat

HPA004765, Anti-NGFR antibody produced in rabbit

Array

CSAA1, Panorama^® Antibody Microarray - Cell Signaling Kit

XP725, Panorama^® Antibody Microarray - XPRESS Profiler725 Kit

Related Categories

... Antibodies for Apoptosis > Antibodies to Death Receptors